Pemanfaatan Nematoda Entomopatogen Steinernema SP. Isolat Lokal Terhadap Mortalitas Penggerek Buah Kopi, H. Hampei Ferr. Di Lapangan.

Potensi nematoda entomopatogen Steinernema sp. terhadap penggerek buah kopi, Hypothenemus hampei di Sumatera Utara belum pernah dilaporkan. Untuk itu telah dilakukan uji mortalitas Steinernema sp. isolat lokal terhadap H. hampei di lapangan. Penelitian bertujuan untuk mendapatkan konsentrasi Steinernema sp. isolat lokal yang tepat terhadap H. hampei di lapangan. Penelitian dilaksanakan pada Bulan April 2015 di kebun kopi petani di Kecamatan Habinsaran Kebupaten Toba Samosir Provinsi Sumatera Utara. Perlakuan yang dicobakan adalah kerapatan nematoda dengan tingkat konsentrasi 5000, 5500, 6000, 6500 ji/ml air steril dan kontrol (tanpa perlakuan). Aplikasi dilakukan dengan menyemprotkan suspensi ke buah-buah kopi matang yang terserang H. hampei. Perbanyakan nematoda dilakukan secara in vivo menggunakan ulat hongkong (Tenebrio molitor). Hasil penelitian menunjukkan bahwa mortalitas larva H. hampei tertinggi terdapat pada perlakuan 6500 ji/ml air steril sebesar 33,13, 33,29 dan 39,53 %. Mortalitas H. hampei meningkat seiring dengan bertambahnya kerapatan nematoda

PENGGUNAAN STYROFOAM SEBAGAI SUBSTITUSI PARSIAL AGREGAT KASAR TERHADAP NILAI KUAT TEKAN DAN KUAT TARIK BELAH BETON RINGAN

Beton merupakan bahan kontruksi suatu bangunan yang paling sering digunakan oleh masyarakat saat ini. Namun beton memiliki kelebihan dan kekurangan. Salah satu kekurangan yang dimiliki beton adalah berat jenis yang besar. Penambahan Styrofoam dalam campuran beton akan membentuk rongga sehingga mengurangi berat beton dan akan membentuk beton ringan den Beton merupakan bahan kontruksi suatu bangunan yang paling sering digunakan oleh masyarakat saat ini. Namun beton memiliki kelebihan dan kekurangan. Salah satu kekurangan yang dimiliki beton adalah berat jenis yang besar. Penambahan Styrofoam dalam campuran beton akan membentuk rongga sehingga mengurangi berat beton dan akan membentuk beton ringan dengan berat volume ≤1900 kg/m3 . Penelitian ini dilakukan untuk mengetahui pengaruh dari penggunaan Styrofoam sebagai subsitusi parsial agregat kasar terhadap kuat tekandan kuat tarik belah. Untuk menghitung komposisi campuran menggunaan ACI.211.1-91. Penilitian ini menggunaka benda uji silinder dengan diameter 100 mm dan tinggi 200 mm. Pengujian dilakukan pada umur beton 7 hari, 14 hari dan 28 hari. Dengan variasi 0%, 60%, 70% dan 80%. Hasil penelitian menunjukan bahwa setiap substitusi stytrofoam pada campuran beton mengakibatkan penurunan kuat tekan dan kuat tarik belah beton, namun pada berat volume, subtitusi Styrofoam sebanyak 60%,70% dan 80% dapat menjadikan berat volume mencapai spesifikasi beton ringan dengan berat volume secara berturut-turut 1792,377 kg/m3, 1734,211 kg/m3, 1618,220 kg/m3. Kata kunci: Beton Ringan, Styrofoam, Kuat Tekan, Kuat Tarik Belah

Tradisi Masyarakat Dalam Penanaman Dan Pemanfaatan Tumbuhan Obat Lekat Di Pekarangan

Home yard has been used to plant certain medicinal plants, also as known as TOGA program. This research was aimed to study the implementation of TOGA program and community tradition in planting and using medicinal plants. A qualitative research was conducted in West Java, Central Java, East Java and Bali which based on National Basic Health Research 2010 data had the highest percentage of jamu USAge. Data was collected in 2011 through in depth interview and focus group discussion with related stakeholder and observation in research location. Result in Bogor district showed TOGA program has been included in Family Welfare Development Program and similar program was also developed in some villages. There was no certain TOGA program in Karanganyar, Sumenep and Gianyar districts. TOGA program in Karanganyar was inserted in other program due to economic needs while in Gianyar the program was originated from ornamental plants. The community usually used medicinal plants for the prime medication before going to health facility. Planting medicinal plants is an inherited tradition where parents become the main source of planting and USAge information. The Agriculture Office and village officials had more significant roles to guide the community while the Health office only suggested or monitored the

Real Time Reasoning in OWL2 for GDPR Compliance

This paper shows how knowledge representation and reasoning techniques can be used to support organizations in complying with the GDPR, that is, the new European data protection regulation. This work is carried out in a European H2020 project called SPECIAL. Data usage policies, the consent of data subjects, and selected fragments of the GDPR are encoded in a fragment of OWL2 called PL (policy language); compliance checking and policy validation are reduced to subsumption checking and concept consistency checking. This work proposes a satisfactory tradeoff between the expressiveness requirements on PL posed by the GDPR, and the scalability requirements that arise from the use cases provided by SPECIAL's industrial partners. Real-time compliance checking is achieved by means of a specialized reasoner, called PLR, that leverages knowledge compilation and structural subsumption techniques. The performance of a prototype implementation of PLR is analyzed through systematic experiments, and compared with the performance of other important reasoners. Moreover, we show how PL and PLR can be extended to support richer ontologies, by means of import-by-query techniques. PL and its integration with OWL2's profiles constitute new tractable fragments of OWL2. We prove also some negative results, concerning the intractability of unrestricted reasoning in PL, and the limitations posed on ontology import

Rapid Identification of Fluorochrome Modification Sites in Proteins by LC ESI-Q-TOF Mass Spectrometry

Conjugation of either a fluorescent dye or a drug molecule to the ε-amino groups of lysine residues of proteins has many applications in biology and medicine. However, this type of conjugation produces a heterogeneous population of protein conjugates. Because conjugation of fluorochrome or drug molecule to a protein may have deleterious effects on protein function, the identification of conjugation sites is necessary. Unfortunately, the identification process can be time-consuming and laborious; therefore, there is a need to develop a rapid and reliable way to determine the conjugation sites of the fluorescent label or drug molecule. In this study, the sites of conjugation of fluorescein-5′-isothiocyanate and rhodamine-B-isothiocyanate to free amino groups on the insert-domain (I-domain) protein derived from the α-subunit of lymphocyte function-associated antigen-1 (LFA-1) were determined by electrospray ionization quadrupole time-of-flight mass spectrometry (ESI-Q-TOF MS) along with peptide mapping using trypsin digestion. A reporter fragment of the fluorochrome moiety that is generated in the collision cell of the Q-TOF without explicit MS/MS precursor selection was used to identify the conjugation site. Selected ion plots of the reporter ion readily mark modified peptides in chromatograms of the complex digest. Interrogation of theses spectra reveals a neutral loss/precursor pair that identifies the modified peptide. The results show that one to seven fluorescein molecules or one to four rhodamine molecules were attached to the lysine residue(s) of the I-domain protein. No modifications were found in the metal ion-dependent adhesion site (MIDAS), which is an important binding region of the I-domain

Pengaruh Stres dalam Pekerjaan terhadap Kinerja Karyawan: Studi Kasus di Perusahaan Agribisnis PT Nic

This study was aimed at finding out the existence of stress in work towards employees' performance, and knowing the indicators of each shaper element of that influencing the employees' performance of plant department of agribusiness industry at PT. NIC. The method of this study is case study involving 155 respondents. Using the Structural Equation Modelling (SEM), it is known that the influence of stress in work towards employees' performance is significantly negative. It means that the advance of stress in work can take the employees' performance down. The advance of stress in work was stimulated by stressor, in this case are job pressure and lack of support. The relationship between stressor and stress in work is positively significant. Lack of support is the most influence indicator of stressor variable instead of job pressure. This study concludes that stress in work significantly influence the employees' performance. Generally, stress in work at plant department of PT. NIC is in low category (41.9%) and the performance is in high/good category (60.6%). It means that the existence of stress in work at this time have positive characteristics because it has played the role as motivator to work